Chen et al., used an organotypic recellulartization model by depleting cells from fresh normal human colon tissue and then seeding in hTERT-immortalized, but otherwise normal, human colonic epithelial cells (hCECs) along with primary myofibroblasts and endoethelial cells from healthy subjects. The SB screen was performed by stably transducing hCECs with an shRNA to APC followed by transfection with a modified version of T2/Onc2 along with SB100x. hCECs were then seeded into the model and monitored for submucosal invasion. 21 invasive neoplasis from 15 recellularized colon matrices were analyzed for SB insertions via linker-mediated PCR followed by sequencing. Insertion sites with the deepest read counts (top 10%) were considered clonal and were considered to identify candidate cancer genes.
Data was extracted from supplemental Table 2. Mouse homologs and GRCm38/mm10 genomic coordinates were identified using MGI Batch Query. Relative rank was assigned based on number of invasive neoplasias with insertions (1 = B, more than 1 = A). Unlike the majority of screens in this database, this was done using human cells and candidate genes were not identified by an anlysis of CISs. Genes were added to the Candidate Cancer Gene Database on March 14, 2017.